Clarity™ Digital PCR Technology
Clarity™ Digital PCR Technology
Developed with the user in mind, the Clarity™ digital PCR system offers a new paradigm in the absolute quantification of target nucleic acids with high precision, accuracy and sensitivity.
The nifty Clarity™ auto loader simplifies one of the most important steps in digital PCR – sample partitioning. Its clever design allows the user to easily load the PCR samples onto the disposable sample loading kit. Once all 8 samples are loaded, the single touch of a button automatically transfers the samples into the tube-strip and uniformly distributes each sample over the chip. The end result is that 8 samples are subdivided into approximately 10,000 partitions each. All these only in about a minute! Truly, sample loading and partitioning for digital PCR have never been easier.
10,000 partitions per tube
8 reactions partitioned per tube-strip within 1 min
Small footprint & lightweight
Performs up to 96 digital PCR reactions per run
The high density chip within the Clarity™ tube-strip makes it possible to have up to 10,000 partitions within the small confines of a 0.2 mL tube. This unique and innovative design means that the partitions on chip are extremely closely packed. Due to its close proximity, it is absolutely critical that these partitions be effectively sealed in order to eliminate cross-contamination. The Clarity™Sealing Enhancer employs a proprietary method to increase the separation between adjacent partitions when subsequently filled with the Sealing Fluid. Without the Sealing Enhancer, eliminating cross-contamination between partitions would be impossible. The Sealing Enhancer is surely the unsung hero within the family.
Eliminates cross-contamination between the high density partitions
Automated, minimal hands on time
8 reactions at a time
Designed to work with the tube-strips, the Clarity™ reader allows fluorescent signals from every single partition of the digital PCR reactions to be detected with high sensitivity. Unlike droplet-based systems, partitions in each tube are detected concurrently, saving time and allowing tube-strips to be read in just minutes. Closed tube format prevents cross contamination among reactions. Walk away operation allows multiple reactions to be read automatically with no user intervention, providing an easy workflow for high throughput digital PCR experiments.
Reads 4 tube-strips or 32 reactions
2-colour detection: FAM and VIC/HEX
Automated, walk away operation
Closed tube system to minimise contamination
Stable partitions minimises sample loss
|Reaction volume||15 µl|
|Partitions per reaction||10,000|
|Partition volume||1.336 nL|
|Reaction per strip||8|
|Tube capacity||0.2 ml|
|Thermal cycling||Deep well thermal cycler for 0.2 ml tubes with adjustable ramp rate
|Chemistries||TaqMan® and SYBR® Green/EvaGreen®
|Detection channels||2-colors: FAM™, VIC/HEX™|
|Detection mode||End point|
|Light source||High power LED|
|Number of LED light source||1|
|Reader interface||USB and Ethernet|
|Auto Loader Dimensions||(W)12 cm x (H)11.5 cm x (D)18 cm, 1 kg|
|Sealing Enhance Dimensions||(W)18 cm x (H)11 cm x (D)29 cm, 4 kg|
|Reader Dimensions||(W)25 cm x (H)42.5 cm x (D)31 cm, 10 kg|