Clarity Plus™ Digital PCR Technology
The Clarity Plus digital PCR system is the latest offering from JN Medsys that offers unprecedented specifications and performance for your absolute quantification needs. With 45,000 partitions per reaction on a high-density chip, it provides higher resolution, sensitivity and dynamic range for your quantifying RNA and DNA. It features a 6-colour detection system that allows for multiplexed quantification in each reaction chip. These represent a significant upgrade from the first generation Clarity system. In spite of these, the Clarity Plus retains the easy workflow and high-throughput capabilities of the first generation system. Taken together, the Clarity Plus has undoubtedly one of the best specifications in any digital PCR system to date. Try it now to feel its power.
Samples are subdivided into more than 40,000 partitions per reaction. Single molecule analysis of these partitions provide higher sensitivity and better accuracy for quantification of DNA/RNA over a wider dynamic range of concentrations.
Absolute quantification of up to 6 targets can be achieved without the need for references and standards. Better quality data can now be attained using fewer reactions and at lower costs. It is now possible to perform high multiplexed digital PCR.
Sample throughput is not compromised. Run up to 96 reactions at once to analyze hundreds of different targets in a single digital PCR run while generating millions of data points. Such performance opens up more applications for digital PCR like never before.
Load and partition
Using the Auto Loader, 8 samples at a time are loaded and pushed into the chips. Each is then subdivided into more than 40,000 high density partitions. There is minimal sample loss and no dead volume.
The Sealing Enhancer increases the separation between adjacent partitions. Addition of the Sealing Fluid seals and discretizes the partitions.
Tube-strips are placed onto a deep 96-well thermal cycler for amplification. Up to 12 tube-strips (or 96 reactions) can be run each time. There is also the flexibility to run fewer strips.
After amplification, tube-strips are placed into the Reader for endpoint signal readout. Multiplexed analysis is achieved with the 6 detection channels that can detect several targets in each chip.
Powered by AI, the software uses a proprietary method to identify every single partition from each chip. Following that, it classifies each partition into positive and negative based on their intensities. With that information and using Poisson statistics, highly multiplex absolute quantification is achieved on the Clarity Plus.
Stable partitions minimises sample loss
|Reaction volume||15 µl|
|Partitions per reaction||40,000|
|Partition volume||0.3 nL|
|Reaction per strip||8|
|Tube capacity||0.2 ml|
|Thermal cycling||Deep well thermal cycler for 0.2 ml tubes with adjustable ramp rate
|Chemistries||TaqMan® and SYBR® Green/EvaGreen®
|Detection channels||6-colors: FAM™, HEX™, ATTO™ 550, Texas Red®, Cy5™, and Cy5.5™
|Detection mode||End point|
|Light source||High power LED|
|Number of LED light source||2|
|Reader interface||USB and Ethernet|
|Auto Loader Dimensions||(W)12 cm x (H)11.5 cm x (D)18 cm, 1 kg|
|Sealing Enhance Dimensions||(W)18 cm x (H)11 cm x (D)29 cm, 4 kg|
|Reader Dimensions||(W)25 cm x (H)42.5 cm x (D)31 cm, 10 kg|