Frequently Asked Question (FAQ)

Digital PCR System

The guidelines to set up our digital PCR experiments are in our user guide. Drop us an email if you are interested at techsupport@jnmedsys.com or contact us directly.
1) Absolute quantification without standard curves 2) Higher relative sensitivity 3) Greater precision 4) More tolerant to PCR inhibitors
Digital PCR can be understood by splitting up the words. "Digital" means that information is binary and "PCR" is polymerase chain reaction. Digital PCR works by using PCR to generate binary data (1 or 0, Yes or No) by end point PCR of individual nanolitre reactions. Poisson formula is then applied on the binary data to get absolute quantification of the target gene.
Yes, you can read the chip prior to amplification. In fact you can read the chip at any time for your research needs. For example, it is possible to run 10 cycles of amplification, read the chip and put it back into the thermal cycler and continue the amplification.
Yes. In fact one of our users have done it in the following publication. https://www.jnmedsys.com/wp-content/uploads/2017/12/Application-of-digital-PCR-with-chip-in-a-tube-format-to-analyze-Adenomatous-polyposis-coli-APC-somatic-mosaicism.pdf
You can check the melting temperature of your primer and probes using online oligo-analyzing tools. After finding the melting temperature, running temperature of 5 degrees Celsius less than the calculated melting temperature would be recommended. If you have to decrease further, do not worry for unspecific homo-dimer or hetero-dimer formations as it would be obvious on our chip and can be easily identified.
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